Lysosomal cysteine proteinases in rat epididymis.

Immunocytochemical localization of lysosomal cysteine and aspartic proteinases, and ubiquitin in rat epidermis.

To analyze the degradation system in epidermal cells during their generation, differentiation, and cell death, immunocytochemical localization of lysosomal cysteine and aspartic proteinases, an endogenous cysteine proteinase inhibitor, cystatin beta, and ubiquitin were examined using rat sole skin. By confocal laser microscopy, granular immunodeposits for lysosomal proteinases were well demonst...

Differential effects of anti-inflammatory agents on lysosomal cysteine proteinases cathepsins B and H from rat spleen.

The reactivity and specificity of commonly used anti-inflammatory agents with lysosomal cysteine proteinases cathepsins B and H purified from rat spleen have been investigated. Of the different agents tested, flufenamic acid and indomethacin were known to be potent inhibitors of cathepsin B. A half-maximal inhibition of the activity of cathepsin B was achieved at drug concentrations of 7.6 X 10...

High-Mr cysteine proteinases from rat skeletal-muscle.

Proteinase Inhibitors: Medical and Biological Aspects (Katunuma, N., ed.), pp. 97-109, Springer-Verlag Co., New York Roisen, F. J., Wilson, F. J., Yorke, G., Inczedy-Marcsek, M. & Hirabayashi, T. (19836) J. Muscle Res. Cell. Motility 4, 163-175 Schwartz, W. N. & Bird, J . W. C. (1977) Biochem. J . 167,811 -820 St. John, A. C., McElligott, M. A., Lee, J. A., Keaton, K. S., Yorke, G . , Roisen, F...

Endogenous inhibitors of lysosomal proteinases.

Specific inhibitors of three lysosomal proteinases are present in the cytosolic and lysosomal compartments of rabbit liver. The cytosolic inhibitors, purified by chromatography on DEAE-Trisacryl and Sephadex G-75, show specificities toward cathepsin M, cathepsins B and L, and fructose 1,6-bisphosphatase converting enzyme (CE), respectively, and are designated IM, IB/L, and ICE. Inhibitors with ...

Lysosomal Cysteine Proteinases as Mediators of Inflammation and Tumor Spread : Control of their Extracellular Proteolytic Activity